Sutural regeneration requires the formation of new connective tissues comprised of various extracellular matrix components. The present study investigated the formation and distribution of the major ECM components such as fibronectin (FN), type I collagen (CI) during expanded sutural regeneration.
The expansion were created on median palatine suture of young adult dog_
At 0 and 60 days after expansion, dogs were sacrificed by perfusion with 10% neutral buffered formalin The sutural area were cut, demineralized, dehydrated and embedded in paraffin. Immunostaining of FN and CI was achieved by the avidin-biotin complex method.
The results were as follows
1. After expansion, much cellular infiltration was observed in the suture. The orientation of the fiber bundles become obscured.
2. At 60 days after expansion, the increase of the fibrous components were seen between both the sutural surfaces. The new bone formation were seen at the regenerated sutural surface.
3. After expansion, intensive staining for FN, CI was observed especially around blood vessels as well as inflammatory cells. This proteins also expressed later in the collagenous bundles.
4. At 60 days after expansion, intensive staining for FN and CI was observed in osteoid, newly formed connective tissue, and its attachment site to the sutural surface.
5. These results suggest that sutural regeneration is achieved through the cell differentiation and formation of Fh and CI by the cells.
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